The main character, whose name has not been released yet, is a recently trained agent of the life saving organization known only as the "Tribulation Knights". A "Tribulation Knight" is an agent of a secret organization created by a now destroyed Christian church association. Their mission is to insure the freedom and safety of the new Christians existing during the time of Tribulation. This period after the rapture known as the Tribulation will be a time where new Christians worldwide will experience persecution for their faith.

The storyline is an interpretation of the Bible concerning the end days. A worldwide cult organization is gaining control of the planet and is planning to incarcerate Christians who do not join them. The Tribulation Knights aim is to prevent that. The missions range from gathering intelligence to releasing fellow operatives from interrogation cells, to saving groups of incarcerated citizens from the clutches of the powerful Enforcers.

Tribulation Knights features stealth as one of the most important and critical aspects of gameplay. What truly sets Tribulation Knights apart from other games on the market are its game rules. Shooting and killing of game characters is absent from this game. A high tech slingshot is one of the primary tools available to a Knight and it is used to take out surveillance and alarm systems. An on-screen partner provides critical information in your time of need.

If the player is detected by any of the various types of surveillance, World Rule Enforcers, or by citizens loyal to the World Order, an alarm will sound attracting unwanted attention. If an alarm has been triggered then the mission might end prematurely, or the NPC enemies can become more aware when triggered. The enemy can capture the knight, which forces the player to replay the level or start again from a remote prison cell. The missions require the player to remain almost invisible to the enemy for most of the game. This requires taking less traveled routes like the ledges of tall buildings, going down dangerous back alleys, crawling through air ducts, and navigating miles of sewer tunnels. Sneak peaks can be found on the website.